microfluidic tissue processor-based (Lunaphore)
Structured Review

Microfluidic Tissue Processor Based, supplied by Lunaphore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/microfluidic tissue processor-based/product/Lunaphore
Average 90 stars, based on 1 article reviews
Images
1) Product Images from "Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections"
Article Title: Microfluidics-assisted multiplexed biomarker detection for in situ mapping of immune cells in tumor sections
Journal: Microsystems & Nanoengineering
doi: 10.1038/s41378-019-0104-z
Figure Legend Snippet: a COC chip with microfluidic channels, a PDMS gasket to create the reaction chamber on top of the tissue sample, and a glass coverslip to enable online imaging of the tissue slide. The exchange of reagents is done in a timeframe of 1 s, following the principle of the fast-fluidic exchange (FFEX) technology. b The LTC is mounted on the stainer and clamped to the glass slide with the tissue by two air-pressure-driven pistons which apply force vertically as indicated by the arrow. It also contains a heating element for precise control of the temperature of the chamber. Furthermore, the stainer can be mounted on a regular motorized microscope stage, thereby enabling the scanning of the tissue slide when clamped with the LTC. c Tissue preprocessing (including dewaxing and antigen retrieval) is performed off-chip. Subsequently, the slide is transferred to the stainer where staining, imaging and elution cycles are performed
Techniques Used: Imaging, Control, Microscopy, Staining
Figure Legend Snippet: a Fluorescence images of biomarkers in tonsils from microfluidic 10-plex IF and bright-field images of conventional single-plex IHC on adjacent slides. Scale bars: 100 µm (overview images) and 15 µm (insets). b Comparison of the stained area fraction from microfluidic multiplex IF and manual single-plex IHC for each marker. The correlation coefficient r and its p value are reported in the same graph
Techniques Used: Fluorescence, Comparison, Staining, Multiplex Assay, Marker